ANTI-BIOFILM ACTIVITY DEMONSTRATED FOLLOWING TREATMENT WITH A NOVEL PLASMA DEVICE
Booth , R., Cutting, K F and westgate, S J
A plethora of data describes the presence of biofilms within chronic wounds and links the presence of biofilm to delayed wound healing. Clinical case studies have demonstrated that the appropriate removal of biofilm can restore the normal progression of healing. This study investigates the efficacy of a novel gas plasma device used to treat Staphylococcus aureus biofilms.
•A twenty-four hour culture of S. aureus was harvested from a Tryptone soya agar (TSA) and re-suspended in Tryptone Soya Broth (TSB).
•The suspension was diluted in TSB to give an overall concentration of 107 cfuml-1 and used as the initial inoculum.
•Polycarbonate coupons were used as the surface for biofilm growth.
•Once prepped, the CDC reactor was incubated for 24 and 72 hours at 37°C with shaking at 50rpm.
• Following incubation coupons were removed from the reactor and washed three times in sterile PBS to remove planktonic organisms.
•Washed coupons were treated with gas plasma for 2 minutes or for 5 minutes. Control coupons were treated with PBS for 5 minutes (N=3).
•Coupons were transferred into 2ml of TSB and sonicated for 5 minutes in order to recover remaining viable microorganisms. Recovered microorganisms were quantified using serial dilutions and colony counts.