In Western countries, prostate cancer (PCa) is the type of cancer with the highest incidence in men. To date, reliable tools for PCa localization are lacking. Recently, contrast-ultrasound dispersion imaging (CUDI) by spatiotemporal analysis performed on transrectal dynamic contrast-enhanced ultrasound (DCE-US) loops has been proposed as a promising option for PCa localization. Until now, CUDI has been performed in 2D only, with each plane requiring a separate contrast-agent bolus injection. 3D DCE-US can potentially solve this limitation, enabling the analysis of the entire prostate with a single bolus injection. Therefore, here we propose CUDI by spatiotemporal similarity analysis in 3D.