251 posters,  10 sessions,  768 authors,  134 institutions

ePostersLive® by SciGen® Technologies S.A. All rights reserved.

Poster 20
OPTIMIZING BIOBANKING PROTOCOLS FOR URINE PROTEIN DETECTION

Primary tabs

Rate

No votes yet

Statistics

640 reads

Optimizing biobanking protocols for urine protein and metabolite detection

Background:

Multiple factors affect the quality of biologic data in urine
These factors include timing of void and duration that urine is kept at room temperature,
In addition, the presence of additives such as bacteriostatic agents (boric acid) or protease inhibitors can affect the proteins and metabolites recovered
 
Objective:

To identify the optimal urine processing protocol that allows for the highest protein yields with minimal degradation.

Methods:

Recruited 10 Caucasian women (ages 35-65) to give multiple urine samples on the same day:
Home collection kits given; collected 1st AM void and placed into aliquots of: 1) no additive; 2) boric acid (BA)
Came to clinic to provide a “random” AM void and urine placed into the same aliquots plus those with protease inhibitor (PI) or combined BA+PI
Urine samples either immediately cooled to 4C or left at room temp (RT)
Proteins quantified using Bradford assay and yields compared across conditions
Sample pools from each condition were analyzed using mass spectrometry to assess for degradation

Conclusions:

For translational studies of urine proteins, there are no significant benefits to obtaining 1st morning void samples
To optimize protein yields in studies of urine, we suggest immediately refrigerating samples
The addition of a protease inhibitor increases protein yields, regardless of storage temperature
Enter Poster ID (e.gGoNextPreviousCurrent